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Registros recuperados: 273 | |
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Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre. |
Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different... |
Tipo: Text |
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF |
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Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre. |
In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat... |
Tipo: Text |
Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf |
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Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie. |
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente... |
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Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf |
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Avia, Komlan; Coelho, Susana M.; Montecinos, Gabriel J.; Cormier, Alexandre; Lerck, Fiona; Mauger, Stephane; Faugeron, Sylvain; Valero, Myriam; Cock, J. Mark; Boudry, Pierre. |
Deciphering the genetic architecture of adaptation of brown algae to environmental stresses such as temperature and salinity is of evolutionary as well as of practical interest. The filamentous brown alga Ectocarpus sp. is a model for the brown algae and its genome has been sequenced. As sessile organisms, brown algae need to be capable of resisting the various abiotic stressors that act in the intertidal zone (e.g. osmotic pressure, temperature, salinity, UV radiation) and previous studies have shown that an important proportion of the expressed genes is regulated in response to hyposaline, hypersaline or oxidative stress conditions. Using the double digest RAD sequencing method, we constructed a dense genetic map with 3,588 SNP markers and identified 39... |
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Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48779/49186.pdf |
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Azema, Patrick; Lamy, Jean-baptiste; Boudry, Pierre; Renault, Tristan; Travers, Marie-agnes; Degremont, Lionel. |
Background In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory... |
Tipo: Text |
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Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00372/48350/48518.pdf |
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Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre. |
The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors. |
Tipo: Text |
Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf |
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Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. |
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... |
Tipo: Text |
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf |
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Batista, Frederico; Ben-hamadou, Radhouan; Fonseca, Vera; Taris, Nicolas; Ruano, Francisco; Reis-henriques, Maria; Boudry, Pierre. |
The taxonomic status of the cupped oysters Crassostrea angulata and C. gigas has received considerable attention in the last decades. Based on larval shell morphology, experimental hybridization, allozymes and nuclear DNA studies several authors have considered these two taxa as being synonymous. However, mitochondrial data showed clear genetic differences between the two taxa. In addition, microsatellite-based studies and cytogenetic studies have also provided evidence that supports their differentiation. Considerable differences have also been observed at the phenotypic level in terms of growth rate and ecophysiological parameters. In the present study, C. angulata from Sado estuary (Portugal) and C. gigas from Seudre estuary (France) were collected and... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Crassostrea angulata; Hybridization; Shell pigmentation; Shell morphology. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3904.pdf |
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Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C.. |
The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas... |
Tipo: Text |
Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf |
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Batista, Frederico; Fonseca, Vera; Ben-hamadou, Radhouan; Taris, Nicolas; Henriques, Maria; Boudry, Pierre. |
Unlike in some crops, no large heterotic effects have been observed in most farmed animals. Interestingly, significant hybrid vigour for some traits has been reported in the Pacific oyster, Crassostrea gigas. Crossbreeding between closely related taxa can be seen as an efficient way to exploit the dominance component of the genetic variance of a trait. Factorial crosses between the Portuguese oyster C. angulata (from Sado estuary, Portugal) and C. gigas (from Seudre estuary, France) were done to evaluate the aquaculture potential of hybrids. Juveniles of the different progenies were reared in Ria Formosa (Portugal) under usual farming conditions. The genetic confirmation of all progenies was done using mitochondrial and nuclear markers in order to detect... |
Tipo: Text |
Palavras-chave: Hybridization; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3429.pdf |
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Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre. |
The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between... |
Tipo: Text |
Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf |
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Batista, Frederico M.; Lopez-sanmartin, Monserrat; Boudry, Pierre; Navas, Jose I.; Ruano, Francisco; Renault, Tristan; Fonseca, Vera G.; Leitao, Alexandra. |
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid... |
Tipo: Text |
Palavras-chave: Aneuploidy; Ostreid herpesvirus 1; Oyster; Crassostrea. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00215/32581/35759.pdf |
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Baud, Jean-pierre; Palvadeau, Hubert; Nourry, Max; Haure, Joel; Penisson, Christian; Boudry, Pierre. |
La production française d' huître creuse Crassostrea gigas représente, avec 144000 tonnes de production, la 4ème' place au ni veau mondial après la Chine (2 285 000 t), le Japon (225 000t.) et la Corée (1 85 000t.). Ce bivalve filtreur possède un potentiel d'adaptation élevé puisqu'on le trouve sous des latitudes très diverses. Malgré son importance économique mondiale (2 900 000 tonnes de production en 1996), à ce jour, très peu de programmes de sélection ont été mis en oeuvre sur cette espèce, qui n'est pas considérée comme domestiquées au sens génétique du terme. Toutefois, avant de lancer des programmes - nécessairement de grande ampleur - pour la sélection de souches d' huîtres creuses Crassostrea gigas, il est utile de répondre au préalable à... |
Tipo: Text |
Palavras-chave: Génétique; Variabilité génétique; Croissance; Huîtres creuses; Crassostrea gigas. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00048/15881/13303.pdf |
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Bayne, B. L.; Ahrens, M.; Allen, S. K.; D'Auriac, M. Angles; Backeljau, T.; Beninger, P.; Bohn, R.; Boudry, Pierre; Davis, J.; Green, T.; Guo, X.; Hedgecock, D.; Ibarra, A.; Kingsley-smit, P.; Krause, M.; Langdon, C.; Lapegue, Sylvie; Li, C.; Manahan, D.; Mann, R.; Perez-paralle, L.; Powell, E. N.; Rawson, P. D.; Speiser, D.; Sanchez, J. -l.; Shumway, S.; Wang, H.. |
Tipo: Text |
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Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00418/52944/53886.pdf |
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Bernard, Ismael; Duchene, Julie; Hily, Christian; Lazure, Pascal; Pouvreau, Stephane; Boudry, Pierre. |
En complément du rapport final (Anonyme (2014). PERLE. Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis. Rapport final de l'ensemble du projet 2011-2014. http://archimer.ifremer.fr/doc/00248/35932/), ce rapport présente l'étude de la phase de dispersion des larves en rade de Brest et analyse le lien entre la quantité de larves émises et la quantité de larves captées. Ce lien, qui possède une forte composante spatiale, est appelé connectivité. Il est sous l’influence de la dispersion et de la survie des larves mais seule la dispersion larvaire, avec différents forçages biophysiques du milieu, a été considérée ici. Les effets de la localisation des zones d’émission et de captage, du comportement des larves, du vent ou de la durée... |
Tipo: Text |
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Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00251/36233/34793.pdf |
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Bernard, Ismael; Lapegue, Sylvie; Dumas, Franck; Talarmain, Eric; Cornette, Florence; Degremont, Lionel; Chavanne, Herve; Pouvreau, Stephane; Auby, Isabelle; Maurer, Daniele; Plus, Martin; Le Moine, Olivier; Boudry, Pierre. |
La forte augmentation des mortalités de juvéniles d’huître creuse depuis 2008 a relancé la problématique de l’approvisionnement en naissains de l’ostréiculture française. Dans ce contexte, cette étude a pour objectif d’étudier la faisabilité d’opérations de repeuplement en huîtres génétiquement plus résistantes, afin d’améliorer les performances de survie du naissain capté dans le milieu naturel. Cette étude s’est ainsi focalisée sur les deux bassins naisseurs historiques, Marennes-Oléron et Arcachon. Nous avons donc cherché à établir (1) une hiérarchie des bancs en fonction de l’expulsion de larves d’huître dans le bassin d’Arcachon, (2) une cartographie des liens en termes de connectivité entre les bancs des pertuis charentais et (3) la faisabilité du... |
Tipo: Text |
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Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19410/17013.pdf |
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Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice. |
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang & Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a... |
Tipo: Text |
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Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf |
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Registros recuperados: 273 | |
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